| Termin | Gospodarz | Gość | Wynik |
|---|---|---|---|
| 31.08.16 18:00 | Caudal Deportivo | Burgos CF | 2:1 |
| 31.08.16 18:00 | Andorra CF | CF Hercules | 0:5 |
| 31.08.16 19:00 | CA Cirbonero | SD Ponferradina | 6:5p.k. |
| 31.08.16 20:00 | Sestao River Club | UD Villa Santa Brigida | 4:2 |
| 31.08.16 20:00 | Atletico Saguntino | SD Formentera | 4:5p.k. |
| 31.08.16 20:00 | CD Boiro | CD Guijuelo | 1:3p.d. |
| 31.08.16 20:00 | SD Amorebieta | UD Socuellamos | 2:1p.d. |
| 31.08.16 20:30 | CD Laredo | Cultural y Deportiva Leonesa | 2:3p.d. |
| 31.08.16 20:30 | CD Calahorra | UD Logrones | 3:1p.k. |
| 31.08.16 20:30 | Barakaldo CF | SD Zamudio | 4:0 |
| 31.08.16 20:30 | Club Lleida Esportiu | AE Prat | 1:0 |
| 31.08.16 20:45 | Lorca Deportiva CF | La Hoya Lorca | 1:2p.d. |
| 31.08.16 20:45 | UE Cornella | San Sebastian Reyes | 2:1p.d. |
| 31.08.16 21:00 | Racing Club de Ferrol | Zamora CF | 3:2 |
| 31.08.16 21:00 | UD Extremadura | Atl Mancha Real | 5:0 |
| 31.08.16 21:00 | Albacete Balompie | Real Union Club | 2:1 |
| 31.08.16 21:30 | CD Toledo Sad | Ub Conquense | 2:0 |
| 31.08.16 21:30 | Atletico Sanluqueno | Real Murcia | 1:0 |