| Termin | Gospodarz | Gość | Wynik |
|---|---|---|---|
| 24.04.21 01:30 | Sporting Kansas City | Orlando City | 1:1 |
| 24.04.21 19:00 | New York City FC | Cincinnati FC | 5:0 |
| 24.04.21 20:00 | Nashville SC | CF Montreal | 2:2 |
| 24.04.21 21:00 | Toronto FC | Vancouver Whitecaps | 2:2 |
| 24.04.21 21:30 | San Jose Earthquakes | FC Dallas | 3:1 |
| 25.04.21 00:00 | Los Angeles FC | Seattle Sounders | 1:1 |
| 25.04.21 02:00 | New England Revolution | D.C. United | 1:0 |
| 25.04.21 02:00 | Philadelphia Union | Inter Miami CF | 1:2 |
| 25.04.21 02:00 | Atlanta United FC | Chicago Fire | 3:1 |
| 25.04.21 02:00 | Minnesota United FC | Real Salt Lake | 1:2 |
| 25.04.21 03:00 | Colorado Rapids | Austin FC | 1:3 |
| 25.04.21 04:30 | Portland Timbers | Houston Dynamo | 2:1 |
| 25.04.21 23:30 | Los Angeles Galaxy | New York Red Bulls | 3:2 |